Fifteen of sixteen cancer cell lines … While the presence of surface markers selectively expressed on CSCs are used to isolate these cells, no marker or pattern of makers are known to prospectively identify CSCs in many tumor types.
419:728–734. (A) Hoechst… Side population (A) and non-side population cells (B) examined using optical microscopy (magnification,… Side population and non-side population cells examined using fluorescence microscopy (magnification, ×100). In such cases exploitation of stem cell characteristics can be used to identify CSCs and one such characteristic is the capacity to extrude dyes such as Hoechst 33342. In such cases exploitation of stem cell characteristics can be used to identify CSCs and one such characteristic is the capacity to extrude dyes such as Hoechst 33342.
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(B) SP cells demonstrated weak signals.ABCG2 expression in xenograft tumor tissues examined by immunohistochemistry (magnification, ×400).
(B) Non-side population xenograft. Please enable it to take advantage of the complete set of features!
Given the cancer stem cell hypothesis, it would be interesting to postulate that the percentage of SP cells within a given cancer cell line would correlate to its tumorigenicity and/or its … (A) Side population xenograft. Lung cancer is among the most lethal malignancies with a high metastasis and recurrence rate. Here, we explored whether human pancreatic ductal adenocarcinoma (PDAC) contains a SP, and whether its gene expression profile is associated with chemoresistance, CSC and prognosis.
The side population of cancer stem-like cells in human oral cancer Cancer initiation and development involves a multistep process speculated to be advanced by genetic instability and/or environ-
33342 dye and are referred to as the ‘‘side population’’ (SP). Cancer cell lines are essential platforms for performing cancer research on human cells. However, SP cells have never been identified or isolated from gastrointestinal cancers. Fifteen of sixteen cancer cell lines from the gastrointestinal system contained 0.3%–2.2% SP cells. COVID-19 is an emerging, rapidly evolving situation. (A)… ABCG2 expression in xenograft tumor tissues examined by immunohistochemistry (magnification, ×400). In this study, we used flow cytometry and Hoechst 33342 dye efflux assay to isolate and characterize SP cells from six human lung cancer cell lines (H460, H23, HTB-58, A549, H441, and H2170). By continuing you agree to the Copyright © 2020 Elsevier B.V. or its licensors or contributors. Cell that exclude this dye are referred to as side population (SP) cells. These cells share characteristics of CSCs, specifically, they are enriched for tumor initiating capacity, they express stem-like genes, and they are resistant to chemotherapeutic drugs.
A subset of stem cells, termed "side population" (SP) cells, has been identified and characterized in several mammalian tissues and cell lines.
Li XX, Wang J, Wang HL, Wang W, Yin XB, Li QW, Chen YY and Yi J: Characterization of cancer stem-like cells derived from a side population of a human gallbladder carcinoma cell line, SGC-996.
While the presence of surface markers selectively expressed on CSCs are used to isolate these cells, no marker or pattern of makers are known to prospectively identify CSCs in many tumor types. If you do not receive an email within 10 minutes, your email address may not be registered, Dye exclusion is a valuable technique as it identifies a unique population of cells with stem-like characteristics.We use cookies to help provide and enhance our service and tailor content and ads. Nonobese diabetic/severe combined immunodeficiency xeno- The present study aimed to isolate and characterize side population (SP) cells in the human lung cancer A549 cell line, and elucidate the molecular mechanism of SP cells underlying lung cancer. We used flow cytometry and the DNA-binding dye Hoechst 33342 to isolate SP cells from various human gastrointestinal system cancer cell lines.
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Stem cells have been isolated by their ability to efflux Hoechst 33342 dye and are referred to as the “side population” (SP). Please check your email for instructions on resetting your password. High prevalence of side population in human cancer cell lines.
We here demonstrate that, across tumor entities, human cancer cell lines harbor minority populations of putative stem-like cells, molecularly defined by dye extrusion resulting in the side population phenotype. Maximilian Boesch 1,2, Alain G. Zeimet 3, Heidi Fiegl 3, Barbara Wolf 3, Julia Huber 2, However, there is no report on isolation of SP cells from human Name must be less than 100 characters ScienceDirect ® is a registered trademark of Elsevier B.V.Copyright © 2008 Elsevier Ireland Ltd. All rights reserved.ScienceDirect ® is a registered trademark of Elsevier B.V. We used flow cytometry and the DNA‐binding dye Hoechst 33342 to isolate SP cells from various human gastrointestinal system cancer cell lines. Identification of Cancer Stem Cell–Like Side Population Cells in Human Nasopharyngeal Carcinoma Cell Line Jing Wang , Li-Ping Guo , Li-Zhen Chen , Yi-Xin Zeng and Shih Hsin Lu Cancer Res April 15 2007 (67) (8) 3716-3724; DOI: 10.1158/0008-5472.CAN-06-4343 Side population (SP) is identified as cells capable of excluding the fluorescent Hoechst dye and anticancer drugs, and represents hematopoietic stem cells and chemoresistant cells from several solid tumors. Herein, we used Hoechst 33342 dye efflux assay and flow cytometry to isolate and characterize the side population (SP) cells from human lung cancer cell line … Cancer stem cells (CSCs) are found in multiple tumor types.
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Side Population Cells.
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