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tC�V..����D#��^�k#H%�2l�H�� �� %PDF-1.3 The ReproRNA™-OKSGM Kit includes ReproRNA™-OKSGM vector, Recombinant B18R Protein, ReproRNA™ Transfection Reagent, and ReproRNA™ Transfection Supplement. This technical video demonstrates how to reprogram fibroblasts in ReproTeSR™ medium using ReproRNA™-OKSGM. Originally developed by Junying Yu and James Thomson(1) and furth 86�`O�� �`�ɵں^[W��j�F�xrȀR,AZur��n���|��������"�DNj�h��ߝ|����^���Ôz�хx�q��e����)!|�'��y(�;��ݭ\`y�E'��'�XW6����[�Rޝ�a>"��U�5�Si�9�d�� Notably, ReproRNA™-OKSGM Kit (Catalog #05930) is a newly launched kit by STEMCELL Technologies. Xo)� �*��5��4����pLò^'}D�q�NQN� �����v>�ص����� Z�6���d��vH5�T8LL�x�b�!`�7�
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Upon signature of an informed consent, dermal fibroblasts were derived from skin biopsy of a 3-year-old female carrying the mutation. �s��*��+�t=����#o�� We generated patient-derived iPSC line (HUi001-A) using self-replicating RNA reprogramming vector, ReproRNA™-OKSGM Kit (STEMCELL Technologies) which was followed by isolation and expansion as individual clone ().Heterozygous substitute (A>T at nucleotide 787) in the PSEN2 gene resulting to N141I missense mutation was confirmed and was consistent with the … %�쏢 ReproRNA™-OKSGM is a non-integrating, self-replicating RNA reprogramming vector for generating human induced pluripotent (iPS) cells. ���R���ڊ���1�amS���Y!bʀ8BT>1���ˢ̨�`ͳ=l��A���B
��#$@ ReproRNA™-OKSGM vector (Catalog #05931) and Recombinant B18R Protein (Catalog #78075) are also available for individual sale. ReproRNA™-OKSGM Reprogramming Vector for Human iPS Cell Generation by STEMCELLTechnologies.
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