2009; 4: 301-12. �9����=C��O -�J�T=�ܷs�`�ɢ¢���bô��5s���b�� You can also search for this author in Over a Human clinical-grade parthenogenetic ESC-derived dopaminergic neurons recover locomotive defects of nonhuman primate models of Parkinson’s disease. We conducted the DAP induction process in 25 randomly chosen vials from MCB003 (one time for each vial). 1541 0 obj <>/Filter/FlateDecode/ID[<20AABBCA5E34F7B152484FADD560C593><9AD363D72B53F848971989F2F1393AEF>]/Index[1517 63]/Info 1516 0 R/Length 118/Prev 7573827/Root 1518 0 R/Size 1580/Type/XRef/W[1 3 1]>>stream 0000004836 00000 n %%EOF There are two sources for human pluripotent stem cells: embryonic stem cells (ESCs) derived from surplus blastocysts created for in vitro fertilization and induced pluripotent stem cells (iPSCs) generated by reprogramming of somatic cells. 0000013818 00000 n
43. The other authors declare no competing interests. Zhang Y, Jiang M, Kim E, et al. 0000004883 00000 n
To examine the variation of the gene expressions among the six independent preparations, we performed a single-cell RT-qPCR analysis.
When the DAPs are transplanted into the striatum of 6-OHDA-lesioned rats, the animals show behavioral improvement. Doi, D. et al. 0000156911 00000 n Induced Pluripotent Stem Cells (iPSCs) are self renewable and can differentiate to different types of adult cells, which has shown great promises in the field of regenerative medicine. Induced pluripotent stem cells (iPSCs) are adult cells that have been made to resemble ESCs. We repeated DAP induction six times and compared the results of whole-genome sequencing (WGS) and whole-exome sequencing (WES) obtained from original peripheral blood cells, undifferentiated iPSCs, and differentiated cells on days 12 and 26.We extensively investigated mutations in the 686 cancer-related genes listed in the Catalog of Somatic Mutations In Cancer (COSMIC) Census (COSMIC version 83). xref \ ��*��s��F��C�̺"kvl`} a����������.�_�� h�"�KW>0�2(�0r0�1dV0�t����M�=������@C��� �/��epaa���6��r�����ӧ���N/�����lBݑ���\��[]q�[��L8�9�5\�Y��rl����lb ��cg���E�b$� ��40��j�q��i< e�\(ld-0F;� Transplantation of embryonic dopamine neurons for severe Parkinson’s disease. Isolation of human induced pluripotent stem cell-derived dopaminergic progenitors by cell sorting for successful transplantation. After exporting the methylation data by using GenomeStudio (V2011.1) and normalizing the beta values by using BMIQResidual plasmids were tested by quantitative PCR with TaqMan™ Gene Expression Master Mix (Thermo Fisher). 0000009028 00000 n Wang, K. et al. Induced pluripotent stem cell (iPS cell), immature cell that is generated from an adult (mature) cell and that has regained the capacity to differentiate into any type of cell in the body. 2010; 6: 71-9. 0000019845 00000 n Regarding donor eligibility for iPSCs, a medical interview and blood exam for contamination were conducted. Extensive graft-derived dopaminergic innervation is maintained 24 years after transplantation in the degenerating parkinsonian brain. 0000156660 00000 n Teschendorff, A. E. et al.
You can also search for this author in designed the study, collected and assembled the data, performed the data analysis and interpretation, and wrote the manuscript. Doi, D., Kikuchi, T., Morizane, A. These factors were chosen because they were known to be involved in the maintenance 0000164175 00000 n are employees of Sumitomo Dainippon Pharma Co., Ltd. H.M. is an employee of Shin Nippon Biomedical Laboratories. Previously, we found that SOX1+PAX6+ cells exist in the early stage of differentiation and that these cells form rosettes and proliferate in the brainTumorigenicity studies in vivo are also not standardized. Cluster generation was done with the HiSeq PE Cluster Kit v4-cBot (illumina) using illumina cBot. They also have been investigating the use of iPS cells in the treatment of certain diseases. 1 Scarless Enriched selection of Genome edited Human Pluripotent Stem Cells 2 Using Induced Drug Resistance 3 Running title: Efficient scarless selection of edited hPSCs 4 Keun-Tae Kim1*, Ju-Chan Park2*, Haeseung Lee3, Hyeon-Ki Jang4, Yan Jin5, Wankyu 5 Kim3, Jeongmi Lee5, Hyongbum Henry Kim6, Sang-Su Bae4,7 and Hyuk-Jin Cha2# 6 0000011680 00000 n The stained samples were visualized by using a fluorescence microscope (BZ-X710; Keyence) and a confocal laser microscope (Fluoview FV1000D; Olympus).
0000007401 00000 n Li, W. et al.
You can also search for this author in A chemical The usability of human induced pluripotent stem cell (iPSC) technology has revolutionized modern medicine. The COSMIC Cancer Gene Census: describing genetic dysfunction across all human cancers.
Physician.
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