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hoechst 33342 protocol

Hoechst 33342 is a cell-permeable fluorescent compound that is able to stain the DNA of eukaryotic and prokaryotic cells by binding with high affinity to the minor groove of AT-rich DNA sequences. 180 0 obj <> endobj Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain (CAS 23491-52-3), with >98% purity. HO protocol 1 Goodell, M.A. �`��1���iY ���� �����x���1���U�]4���.3�0$2g^Ƽ��3W�K��!�B�63�qs2p2�2K3�2H0f��'���Z�������Ac`� 0000002737 00000 n These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33,342nd compound made by the company. 0000002122 00000 n 0000014722 00000 n Aim.

0000060254 00000 n 301 33 Final concentration should be 2

There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. 0000004043 00000 n H��TMo1�ﯘ�]���Q��)�ծ�C�Mh ��h��;�(%� 0000004647 00000 n 0000004397 00000 n h�b```�,l� A�i?k�[�R�n�f�(zN``v�4 \f޹0�bJ sC�F�GGCZG�����n�@� ���v�3�3t��8!u��[�}��w]��`I5��@���;pg�30��d ;��\T���C1D-�@� �o' 0000001287 00000 n (1996) J Exp Med 183, 1797-806) The Hoechst purification was established for murine hematopoietic stem cells (HSC) on 0000004870 00000 n 0000007322 00000 n The dyes Hoechst 33258 and Hoechst 33342 are the … This stain can be used on both live and fixed cells (1). %%EOF On binding to DNA, the fluorescence greatly increases. Hoechst 33342 is used for specifically staining the nuclei of living or fixed cells and tissues. 0000053190 00000 n 0000000016 00000 n 0 0000006001 00000 n H��U�N�@}߯�G["�ۋRqPI�����Cj" M���wv֎�*�gbvfΜ=3\�$��m��y�Ћ��˫qNI��V;��Cw՚$����$eɩ��p�9��"�s`����,��\�h2+�o��r�yVи|$OC����X*1�=72,U��/����7 L`�˯���0�e�j��^O����&�1-4�0��Lj�eՖ�\���W�9�h�"�d��xC��+���L���;>�� 5/2&�gr��ݞ�j��6���d�{ٺ���a�\�s imϬB�p�Vq��_��&�~ܺr}�^Ce)O���2���`�#EJm��R�)ƌ�D�V)�h!��J��{�tʧu��l����04{����]C�R��cA� &`�?\y� W����c jE�`�Hq�&}�R���0�ZZ�R�t����i��m4{Y�����Ft\���Yn�4�Y~:��=%��*�B6�2�ֲ2���z· endstream endobj startxref trailer 0000000974 00000 n خ � ̞jd:c�@vơ����l⑎�f� �� �ޜ�Ƿ'��§��]�BGO���0����A!�Q�ӓ�����Դ]����S]���o�z�ѽ����9�ފXF�;,V�s�� \w�"X���[.���z��f���x��9�z�XV��֜��Q�z���'�'�"�~BP��0w�(v�M�AED#�_j�i&����_���06���-)���GɰZ�*��m�*�ڕ�{ C��6��5zG�_ ���4 0000052345 00000 n Hoechst(33342(Staining(for(Cell(Cycle(Analysis(of(Live(Cells(! x�bbb`b``Ń3� ���� � ��� 0000001728 00000 n 0000003650 00000 n

Portugal, J. and Waring, M.J. (1988) Biochim Biophys Acta 949, 158-68. 0000007456 00000 n Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. PureBlu Hoechst 33342 Nuclear Staining Dye is a highly pure formulation of Hoechst 33342 fluorescent dye (Figure 1) packaged in a user-friendly format. 0000010162 00000 n <<8233EB5874F7B1498B08DBED6D2BAF70>]>> endstream endobj 181 0 obj <>>> endobj 182 0 obj <>/ProcSet[/PDF/Text]>>/Rotate 0/StructParents 0/Type/Page>> endobj 183 0 obj <>stream

0000006761 00000 n �{�k��f��5��{f��N�vƘ�\h%ra�r4hqy6����:��B�������E����=\J*�34�PB�yB��"4�ꚝ��³��r��(li�σ4�}2l�)Y�hKS��鲵@����S�č�`HN�TLydc��bA�-�߰ wen��Np �T"m��A`�xa��J��J��(Qi��^!�|N��ݝ���zf�U�Z[�����=e�)O��un���f��n���b��`�B4�w����z�� ?P�ݱ٘��vSM�=�SC�lRf_��߹p�$��Ђu�&- %PDF-1.4 %���� 0 Hoechst 33342 will also work with parafomraldehyde or ethanol-fixed cells with modification to the above protocol. ... Hoechst 33342 *20 mM solution in water* 8/99 Hoechst 33342 HSC Staining and Stem Cell Purification Protocol (see Goodell, M., et al. Two of these closely related bis-benzimides are commonly used: Hoechst 33258 and Hoechst 33342. 0000008204 00000 n The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. 0000003228 00000 n endstream endobj 302 0 obj <>/Metadata 24 0 R/PieceInfo<>>>/Pages 23 0 R/PageLayout/OneColumn/StructTreeRoot 26 0 R/Type/Catalog/LastModified(D:20100514095112)/PageLabels 21 0 R>> endobj 303 0 obj <>/ColorSpace<>/Font<>/ProcSet[/PDF/Text/ImageC/ImageI]/ExtGState<>>>/Type/Page>> endobj 304 0 obj <> endobj 305 0 obj <> endobj 306 0 obj <> endobj 307 0 obj <> endobj 308 0 obj [/ICCBased 324 0 R] endobj 309 0 obj [/Indexed 308 0 R 252 326 0 R] endobj 310 0 obj <> endobj 311 0 obj <> endobj 312 0 obj <> endobj 313 0 obj <> endobj 314 0 obj <> endobj 315 0 obj <>stream 0000012028 00000 n Dyes that bind to DNA, such as Hoechst 33342 or 4',6-diamidino-2-phenylindole (DAPI), can be used to observe nuclear condensation. 0000060022 00000 n Hoechst 33342 (from Sigma) comes as a lyophilized powder and is re-suspended at 1 mg/mL in water, filter sterilized, and frozen in small aliquots.. HBSS+ (Hanks Balanced Salt Solution, Gibco) with 2% Fetal Calf Serum and 10 mM HEPES buffer (Gibco).DMEM+ (Gibco) with 2% Fetal Calf Serum and 10 mM HEPES buffer (Gibco).. Propidium Iodide (PI) from Sigma. 0000005177 00000 n This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture.

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hoechst 33342 protocol