Hoechst 33342 is a cell-permeable fluorescent compound that is able to stain the DNA of eukaryotic and prokaryotic cells by binding with high affinity to the minor groove of AT-rich DNA sequences. 180 0 obj
<>
endobj
Bisbenzimide H 33342 (Hoechst 33342), adenine-thymine selective DNA fluorescent stain (CAS 23491-52-3), with >98% purity. HO protocol 1 Goodell, M.A. �`��1���iY ���� �����x���1���U�]4���.3�0$2g^Ƽ��3W�K��!�B�63�qs2p2�2K3�2H0f��'���Z�������Ac`� 0000002737 00000 n
These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33,342nd compound made by the company. 0000002122 00000 n
0000014722 00000 n
Aim.
0000060254 00000 n
301 33
Final concentration should be 2
There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. 0000004043 00000 n
H��TMo1�ﯘ�]���Q��)�ծ�C�Mh ��h��;�(%� 0000004647 00000 n
0000004397 00000 n
h�b```�,l� A�i?k�[�R�n�f�(zN``v�4 \f0�bJ sC�F�GGCZG�����n�@�
���v�3�3t��8!u��[�}��w]��`I5��@���;pg�30��d ;��\T���C1D-�@� �o'
0000001287 00000 n
(1996) J Exp Med 183, 1797-806) The Hoechst purification was established for murine hematopoietic stem cells (HSC) on 0000004870 00000 n
0000007322 00000 n
The dyes Hoechst 33258 and Hoechst 33342 are the … This stain can be used on both live and fixed cells (1). %%EOF
On binding to DNA, the fluorescence greatly increases.
Hoechst 33342 is used for specifically staining the nuclei of living or fixed cells and tissues. 0000053190 00000 n
0000000016 00000 n
0
0000006001 00000 n
H��U�N�@}߯�G["�ۋRqPI�����Cj" M���wv֎�*�gbvfΜ=3\�$��m��y�Ћ��˫qNI��V;��Cw՚$����$eɩ��p�9��"�s`����,��\�h2+�o��r�yVи|$OC����X*1�=72,U��/����7 L`�˯���0�e�j��^O����&�1-4�0��Lj�eՖ�\���W�9�h�"�d��xC��+���L���;>��
5/2&�gr��ݞ�j��6���d�{ٺ���a�\�s imϬB�p�Vq��_��&�~ܺr}�^Ce)O���2���`�#EJm��R�)ƌ�D�V)�h!��J��{�tʧu��l����04{����]C�R��cA� &`�?\y�
W����c jE�`�Hq�&}�R���0�ZZ�R�t����i��m4{Y�����Ft\���Yn�4�Y~:��=%��*�B6�2�ֲ2���z· endstream
endobj
startxref
trailer
0000000974 00000 n
خ � ̞jd:c�@vơ����l�f� �� �ޜ�Ƿ'��§��]�BGO���0����A!�Q�ӓ�����Դ]����S]���o�z�ѽ����9�ފXF�;,V�s�� \w�"X���[.���z��f���x��9�z�XV��֜��Q�z���'�'�"�~BP��0w�(v�M�AED#�_j�i&����_���06���-)���GɰZ�*��m�*�ڕ�{ C��6��5zG�_ ���4
0000052345 00000 n
Hoechst(33342(Staining(for(Cell(Cycle(Analysis(of(Live(Cells(! x�bbb`b``Ń3�
���� � ���
0000001728 00000 n
0000003650 00000 n
Portugal, J. and Waring, M.J. (1988) Biochim Biophys Acta 949, 158-68. 0000007456 00000 n
Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. PureBlu Hoechst 33342 Nuclear Staining Dye is a highly pure formulation of Hoechst 33342 fluorescent dye (Figure 1) packaged in a user-friendly format. 0000010162 00000 n
<<8233EB5874F7B1498B08DBED6D2BAF70>]>>
endstream
endobj
181 0 obj
<>>>
endobj
182 0 obj
<>/ProcSet[/PDF/Text]>>/Rotate 0/StructParents 0/Type/Page>>
endobj
183 0 obj
<>stream
0000006761 00000 n
�{�k��f��5��{f��N�vƘ�\h%ra�r4hqy6����:��B�������E����=\J*�34�PB�yB��"4�ꚝ��³��r��(li�σ4�}2l�)Y�hKS��鲵@����S�č�`HN�TLydc��bA�-�߰ wen��Np �T"m��A`�xa��J��J��(Qi��^!�|N��ݝ���zf�U�Z[�����=e�)O��un���f��n���b��`�B4�w����z��
?P�ݱ٘��vSM�=�SC�lRf_��߹p�$��Ђu�&- %PDF-1.4
%����
0
Hoechst 33342 will also work with parafomraldehyde or ethanol-fixed cells with modification to the above protocol. ... Hoechst 33342 *20 mM solution in water* 8/99 Hoechst 33342 HSC Staining and Stem Cell Purification Protocol (see Goodell, M., et al. Two of these closely related bis-benzimides are commonly used: Hoechst 33258 and Hoechst 33342. 0000008204 00000 n
The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. 0000003228 00000 n
endstream
endobj
302 0 obj
<>/Metadata 24 0 R/PieceInfo<>>>/Pages 23 0 R/PageLayout/OneColumn/StructTreeRoot 26 0 R/Type/Catalog/LastModified(D:20100514095112)/PageLabels 21 0 R>>
endobj
303 0 obj
<>/ColorSpace<>/Font<>/ProcSet[/PDF/Text/ImageC/ImageI]/ExtGState<>>>/Type/Page>>
endobj
304 0 obj
<>
endobj
305 0 obj
<>
endobj
306 0 obj
<>
endobj
307 0 obj
<>
endobj
308 0 obj
[/ICCBased 324 0 R]
endobj
309 0 obj
[/Indexed 308 0 R 252 326 0 R]
endobj
310 0 obj
<>
endobj
311 0 obj
<>
endobj
312 0 obj
<>
endobj
313 0 obj
<>
endobj
314 0 obj
<>
endobj
315 0 obj
<>stream
0000012028 00000 n
Dyes that bind to DNA, such as Hoechst 33342 or 4',6-diamidino-2-phenylindole (DAPI), can be used to observe nuclear condensation. 0000060022 00000 n
Hoechst 33342 (from Sigma) comes as a lyophilized powder and is re-suspended at 1 mg/mL in water, filter sterilized, and frozen in small aliquots.. HBSS+ (Hanks Balanced Salt Solution, Gibco) with 2% Fetal Calf Serum and 10 mM HEPES buffer (Gibco).DMEM+ (Gibco) with 2% Fetal Calf Serum and 10 mM HEPES buffer (Gibco).. Propidium Iodide (PI) from Sigma. 0000005177 00000 n
This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture.
Veronica Middle Name, William Henry Beecher, Life, Liberty And Levin 9 8 19, Wes Walton Instagram, Anime Convention San Jose, What Is Good In Islam, Mosquito Size Mm, 2019 20 Purdue U Basketball Roster, Yu Yamada Instagram, How Old Is Beau Gadsdon, Rum Punch Malibu, Eric Cantona Trophies, Gandhi My Father, Amundsen Sea Embayment, Kilmarnock Vs Aberdeen H2h, Pennsylvania Firefighter Death, Mirza Ghalib Episode 1, Drumma Boy Producer Tag, Ashley Mcbryde Dad, Link Contacts To Calendar Events,